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Image Search Results
Journal: Frontiers in Immunology
Article Title: Application of spatial transcriptomics analysis using the Visium system for the mouse nasal cavity after intranasal vaccination
doi: 10.3389/fimmu.2023.1209945
Figure Lengend Snippet: Application of the Visium system to assay gene expression in mouse nasal tissues. (A) Spatial transcriptome analysis using the Visium system in bone tissue Left: HE staining of a mouse head in cross-section. Middle: Spots within the area determined to be bone areas based on morphological evaluation of the HE-stained tissue are shown in red. Scale bar = 1 mm. Right: The 25 most expressed genes in the selected spots are shown in the bar graph. Red letters indicate genes related to bone or supporting muscle. Black letters indicate other genes. The bar graph was created using the R statistical software package (version 4.2.2). (B) Spatial transcriptome analysis of the nasal epithelium and lymphoid tissue area using the Visium system. Left: HE staining of a mouse head in cross-section. Scale bar = 1 mm. Middle: Spots within areas that have been morphologically evaluated based on HE-stained tissue sections and determined to contain lymphocytes are shown in red. Right: The 25 most expressed genes in the selected spots are shown in bar graph. Red letters indicate genes that are related to nasal functions (e.g., olfaction). Blue letters indicate genes related to the immune system. Black letters indicate other genes. The bar graph was created using the R statistical software package (version 4.2.2). (C) Chart of areas that have been morphologically evaluated based on HE-stained tissue sections and determined to contain lymphocytes. A total of 18,404 genes were identified as being expressed in nasal epithelium and lymphoid tissue-associated spots based on log2 fold changes and median normalized average. The chart was created using the R statistical software package (version 4.2.2). Horizontal axis: Median normalized average of gene expression levels for each selected spot. Vertical axis: Log2 fold changes in gene expression levels for selected spots. Log2 fold changes ≥ 1 are indicated by yellow dots, log2 fold changes ≤-1 are indicated by violet dots, and all others are indicated by gray dots. Averages of the top five most expressed genes and genes related to olfactory function are indicated by red letters; genes related to immune function are indicated by blue letters. Up, upregulated; Down, downregulated; NS, not significant.
Article Snippet: A volcano-plot was created using the
Techniques: Gene Expression, Staining, Software